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Image Search Results
Journal: NPJ Vaccines
Article Title: Stabilization of norovirus GII.3 virus-like particles by rational disulfide engineering
doi: 10.1038/s41541-025-01254-2
Figure Lengend Snippet: A ELISA plates were coated with 51 human saliva samples and incubated with GII.3 or GII.3-DS1 VLPs at 20 µg/mL. Bound VLPs were detected using polyclonal sera against GII.3. Relative luminescent units (RLU) of GII.3 and GII.3-DS1 are reported on the x- and y -axes, respectively. Data are fitted into a simple linear regression model using GraphPad Prism software. B Plates were coated with 50 ng of either GII.3 or GII.3-DS1 VLP and incubated with 19 unique human monoclonal antibodies starting at 4 µg/mL and diluted in a four-fold, ten-point dilution series. Bound antibodies were detected using goat anti-human Ig-Fc fragment conjugated to horseradish peroxidase (HRP). Interpolated endpoint titer for each antibody against either GII.3 or GII.3-DS1 is reported on the x - and y -axes, respectively. Data are fitted into a simple linear regression model using GraphPad Prism software.
Article Snippet: VLP-bound antibodies were detected with HRP-goat anti-human IgG,
Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Software, Bioprocessing